Coding

Part:BBa_K228863

Designed by: Lin Min   Group: iGEM09_PKU_Beijing   (2009-09-22)

PhiR73 delta with amber mutation

This is the mutated version of P2 Phage PhiR73 activator. The first TCA Serine codon is mutated to a TAG amber stop codon. The translation is ceased unless there is supD tRNA which is a amber mutation suppressor. It is one of the components of Peking 2009 Team AND Gate. This part is modified from BBa_I746352.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aPhiR73 delta with amber mutation